Figure 2: SDS-PAGE fractionation of denuded germinal vesicle (GV) and metaphase II (M II) oocyte proteins. Pooled protein (20 μg of denuded GV or M II oocyte protein) is run on a 12.5% acrylamide gel and then subjected to silver staining. A: Lane 1: total protein of GV oocytes; Lane 2: total protein of M II oocytes. Each gel lane is excised and then subjected to ingel digestion prior to LC/MS-MS analysis. B: 1 442 proteins are found in both the GV and M II stages and 260 proteins were differentially expressed between GV and M II stages.