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Year : 2021  |  Volume : 10  |  Issue : 3  |  Page : 121-126

Effect of royal jelly on in vitro fertilization and early embryo development following nicotine treatment in adult female rats

1 Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
2 Department of Veterinary Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Correspondence Address:
Ali Shalizar-Jalali
Department of Veterinary Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia
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Source of Support: This study was financially supported by Urmia University, Urmia, Iran, as a part of MSc thesis (No. 2.291), Conflict of Interest: None

DOI: 10.4103/2305-0500.316624

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Objective: To scrutinize the protective role of royal jelly as an antioxidant on nicotine-induced changes in malondialdehyde (MDA) level, p53 expression, in vitro fertilization (IVF) rate, and early embryo development in adult female rats. Methods: A total of 56 adult female Wistar rats were divided into 8 groups (n=7 in each group). Group 1 served as an untreated control group, group 2, 3 and 4 received nicotine at a dose of 0.50, 1.00 and 2.00 mg/kg respectively, group 5 received royal jelly at a dose of 100.00 mg/kg, and group 6, 7 and 8 received 0.50, 1.00 and 2.00 mg/kg nicotine, respectively, with 100.00 mg/kg body weight royal jelly. Nicotine and royal jelly were administered daily for 49 days in the experimental groups intra-peritoneally and orally, respectively. At the end of the experimental period, p53 expression, IVF rate and early embryo development as well as MDA concentration were measured. Results: The IVF rate, number of cumulus oocytes, two-cell embryos and blastocysts decreased in the nicotine-treated groups in a dose-dependent manner. In addition, p53 mRNA expression and MDA levels increased in the nicotine-treated groups. Royal jelly co-administration led to partial improvement in the aforementioned parameters. Conclusions: Royal jelly may have a repro-protective effect in nicotine-administered female rats in terms of its anti-oxidant and anti-apoptotic properties.

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