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  Indian J Med Microbiol
 

Figure 5: Effect of methanolic extract of Glyphaea brevis twig (GBT) on necrosis and apoptosis. Representative micrographs revealed by flow cytometry dot plot analysis of annexin-V/PI- labelled TM4 Sertoli cells after 72 h exposure with control (0.2% DMSO) (A), baseline (untreated cells with media only) (B), extract of Glyphaea brevis twig at different concentrations of 1.0 μg/mL (C), 10.0 μg/mL (D), 100.0 μg/mL (E) and 1000.0 μg/mL (F). Data are expressed as mean ± SD of six independent experiments performed in triplicate. *P<0.05, when treated groups are compared with the control

Figure 5: Effect of methanolic extract of <i>Glyphaea brevis</i> twig (GBT) on necrosis and apoptosis. Representative micrographs revealed by flow cytometry dot plot analysis of annexin-V/PI- labelled TM4 Sertoli cells after 72 h exposure with control (0.2% DMSO) (A), baseline (untreated cells with media only) (B), extract of <i>Glyphaea brevis</i> twig at different concentrations of 1.0 μg/mL (C), 10.0 μg/mL (D), 100.0 μg/mL (E) and 1000.0 μg/mL (F). Data are expressed as mean ± SD of six independent experiments performed in triplicate. *<i>P</i><0.05, when treated groups are compared with the control