Figure 3: Flow cytometer analysis of influence of Glyphaea brevis (GBT) twig extracts on mitochondria depolarization in TM4 Sertoli cells (stained wit] TMRE dye). Histograms represent cells with depolarized mitochondria that are harvested from untreated cells (A), cells treated with DMSO (B), cells treatei with treated with DMSO and carbonyl cyanide m-chlorophenylhydrazone (C), cells treated with methanolic extract of Glyphaea brevis twig of 10 μg/ml (D) and 100 μg/mL (E). Values are expressed as mean ± SD, n=6. NC: negative control (untreated), PC: positive control (treated with DMSO and carbony cyanide m-chlorophenylhydrazone); *P<0.05, compared to the negative control. DMSO: dimethylsulfoxide.