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ORIGINAL ARTICLE
Year : 2018  |  Volume : 7  |  Issue : 3  |  Page : 139-142

Effect of different concentration of fish oil in skim milk-egg yolk extenders on post- thawed semen qualities of Kalang swamp buffalo bull


1 Department of Animal Science, Faculty of Agriculture, Islamic University of Kalimantan, Banjarmasin-South Kalimantan, Indonesia
2 Office of Animal Husbandry District of Banjar, South Kalimantan Province, Indonesia
3 Centre of Artifical Insemination, Banjarbaru, South Kalimantan Province, Indonesia

Correspondence Address:
Abdul Malik
Department of Animal Science, Faculty of Agriculture, Islamic University of Kalimantan, Banjarmasin-South Kalimantan
Indonesia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2305-0500.233576

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Objective: To explore the effect of fish oil at different concentrations on post-thawed semen of Kalang swamp buffalo. Methods: A total of 4 Kalang swamp buffalo bulls with 3-5 years of age and weighed about 340-360 kg were slected. Semen was regularly collected from these buffalo bulls once a week by an artificial vagina. Fish oil was supplementary at the dosages of 0 mg (control), 50 mg, 100 mg, 150 mg, and 200 mg to the extender (skim milk-egg yolk). Fresh, pre-freezing and frozen semen were thawed at 37 °C and evaluated for motility, viability, morphology, and plasma integrity of membrane. Results: The study results indicated that before freezing, supplementation of fish oil at the dose of 150 mg in the extender had significantly motility. And a significant (P<0.05) increase was observed in viability and motility of post-thawed semen at the dose of 150 mg fish oil, which was in difference with other treatment groups. Conclusions: Addition of 150 mg fish oil in the extender could be positive for the enhancement of the quality of post-thawed semen of Kalang swamp buffaloes.


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