• Users Online: 534
  • Print this page
  • Email this page
Year : 2018  |  Volume : 7  |  Issue : 2  |  Page : 62-71

Effect of voltage-gated sodium channels blockers on motility and viability of human sperm in vitro

1 Riphah Institute of Pharmaceutical Sciences, Riphah International University Islamabad, Pakistan
2 Laboratory of Cardiovascular and Integrative Pharmacology, College of Pharmacy, University of Sargodha, Pakistan
3 Faculty of Pharmacy, Gomal University, Dera Ismail Khan, Pakistan

Correspondence Address:
Taseer Ahmad
Laboratory of Cardiovascular and Integrative Pharmacology, College of Pharmacy, University of Sargodha
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2305-0500.228015

Rights and Permissions

Objective: To test the effect of voltage-gated sodium channels (VGSCs) blockers on the motility and viability of human sperm in-vitro and to evaluate the tested compounds as potential contact spermicidal. Methods: Sperm samples were obtained from healthy nonsmoking volunteers of age 25-30 years who had not taken any drug 3 months before and during the course of the study. The effect of VGSCs blockers evaluated from two pharmacological classes including antiarrhythmic (amiodarone, procainamide and disopyramide) and antiepileptic (carbamazepine, oxcarbazepine, phenytoin, and lamotrigine) drugs. They were tested on the in-vitro motility and viability of human sperm using Computer Assisted Semen Analyzer. Results: All tested drugs except oxcarbazepine showed dose dependent inhibition of total motility with significant reduction (P<0.05) at the maximum concentration of 200 μΜ when compared with the control. The concentrations of drugs that reduced total sperm motility to 50% of control (half maximal inhibitory concentration) were 2.76, 14.16 and 20.29 μΜ for phenytoin, lamotrigine and carbamazepine, respectively; and 2.53, 5.32 and 0.37 μΜ for amiodarone, procainamide and disopyramide, respectively. The anti-motility effects were reversible to various degrees. There was statistically insignificant difference in the inhibition of sperm viability among amiodarone, procainamide and disopyramide. Phenytoin demonstrated the most potent spermicidal action. Conclusions: VGSCs blockers have significant adverse effects on in-vitro motility of human spermatozoa. So in-vivo studies are required to determine their potential toxicological effects on human semen quality, which is an important factor regarding fertility. Moreover, these drugs have the potential to be developed into contact spermicidal.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded161    
    Comments [Add]    
    Cited by others 1    

Recommend this journal