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ORIGINAL ARTICLE
Year : 2017  |  Volume : 6  |  Issue : 6  |  Page : 279-282

Cryopreservation of cattle semen using coconut water extender with different glycerol concentrations


Animal Reproduction and Al Department, Veterinary Division, National Research Centre, Dokki, Giza, Egypt

Correspondence Address:
Reda Ibrahim El-Sheshtawy
Animal Reproduction and Artificial Insemination Department, Veterinary Research Division, 13 National Research Centre, Dokki, Giza
Egypt
Walid Said El-Nattat
Animal Reproduction and Artificial Insemination Department, Veterinary Research Division, National Research Centre, Dokki
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2305-0500.217343

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Objective: To investigate the effect of coconut water with a lone concentration and different concentrations of glycerol on chilled and cryopreserved cattle semen characteristics. Methods: Semen was collected from five mature cattle bulls, at weekly intervals for 5 weeks. The ejaculates were pooled and evaluated for dilution processing. Tris citrate egg yolk fructose was used as control treatment for semen, while 50% (V/V) coconut water, 25% (V/V) bi-distilled water and 25% (V/V, 5% anhydrous monosodium citrate) to 20 mL egg yolk and three different concentrations of glycerol (4%, 6% and 8%) were used as coconut water (CW)- glycerol-yolk extenders (CWCG-4, CWCG-6 and CWCG-8). Extended semen was cooled and cryopreserved. Sperm motility%, sperm membrane integrity%, normal acrosome%, live sperm% and total sperm abnormalities% were recorded after equilibrium and after freeze-thawing. Results: The addition of 4% glycerol to coconut water enriched media (CWCG-4) revealed the most effective addition of glycerol on all parameters after equilibrium and after freeze-thawing. Conclusions: Coconut water enriched media with 4% glycerol addition is safe to be used as an extender in bull semen preservation because it is a sterile liquid. So, it can be used without addition of antibiotics to the extender, as antibiotics have to some extent hazardous effect on spermatozoa.


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