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ORIGINAL ARTICLE
Year : 2017  |  Volume : 6  |  Issue : 1  |  Page : 18-22

Maternal reproductive health: Expression patterns of antioxidant enzyme selenoproteins of post-implantation embryos conceived by ethanol-treated murine mothers supplemented with α-tocopherol


1 Biology Department, De La Salle University, Taft Avenue, Manila, Philippines, 1004; University Science Foundation-University Research Coordination Office, (USF-URCO), De La Salle University, Manila, Philippines
2 Biology Department, De La Salle University, Taft Avenue, Manila, Philippines, 1004
3 Biology Department, De La Salle University, Taft Avenue, Manila, Philippines, 1004; Molecular Biology Unit, CENSER, De La Salle University, Taft Avenue, Manila, Philippines
4 Department of Biology, College of Arts and Sciences, University of the Philippines- Manila

Correspondence Address:
Gliceria B Ramos
Biology Department, De La Salle University, Taft Avenue, Manila, Philippines, 1004; University Science Foundation-University Research Coordination Office, (USF-URCO), De La Salle University, Manila, Philippines

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Source of Support: None, Conflict of Interest: None


DOI: 10.12980/apjr.6.20170104

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Objective: To investigate if the protective effect of α-tocopherol against the impact of ethanol on brain morphogenesis involved the activity of the selenoproteins phospholipid hydroperoxide glutathione peroxidase (PHGPx; GPx4) and selenoprotein P (SelPP) that have roles against oxidative stress. Methods: Forty female mice were randomly assigned into natural control (CON), positive control (ETOH), low-, medium-, and high-α-tocopherol-supplemented-ethanol groups (LTOC, MTOC, HTOC, respectively). CON received drinking water without ethanol while ETOH, LTOC, MTOC and HTOC groups received 20% ethanol in drinking water. The supplemented groups were given respective dosages of α-tocopherol, 0.410, 0.819, and 1.640 mg/g body weight, at day 14 before mating onwards to the day 9 of gestation. At 10.5 ED of gestation (1 100 h), the pregnant females were sacrificed by cervical dislocation and the embryos were harvested. Total RNA were extracted, cDNA synthesis and qRT-PCR analyses were carried out. Results: The level of expression of PHGPx in the positive control was significantly lower than that of the natural control. Among the three α-tocopherol-supplemented groups, only the medium dose-group was significantly higher than the positive control. The level of expression of SelPP in the positive control was significantly lower than those of the natural control, the low- and medium-dose-tocopherol supplemented groups. In the high dose-α-tocopherol supplemented group, the level of expression was not significantly different from the positive control but significantly lower than the natural control. Conclusions: The activity of the selenoproteins PHGPx and SelPP are involved in the internetwork of antioxidative enzymes with vitamin E when given up to a medium dose only and is one of the possible pathways of shielding embryonic development against the impact of ethanol on brain morphogenesis. This study strengthens the impact of dietary α-tocopherol and Selenium supplement during the critical period of pregnancy.


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